Laboratory of Virology is focused on the study of plant viruses and management of plant viral diseases, biotechnological utilization of plant viruses, metabolic engineering of plant cells and very recently on investigation of influence of abiotic stress on plant virus infection.

Our model plant species are A. thaliana and N. benthamiana and the leguminous crop plants soybean and pea (G. max and P. sativum). We use not only whole plants but also tissue cultures.

For our experiments we use several RNA and DNA plant viruses, namely Potato virus X, Tobacco mosaic virus, Bean yellow dwarf virus and Cowpea mosaic virus.

During the last three years, we have introduced a novel modular cloning strategy based on the GoldenBraid system that allows us to efficiently design genomic elements and complex plant expression cassettes for metabolic engineering of plant cells.


Research areas of the laboratory:

Understanding and management of plant viral diseases

·         Study of molecular properties of plant viruses by immunological and molecular biological methods

·         Genome mapping of plant viruses, viral isolates and phylogenetic analysis

·         Preparation of polyclonal antibodies against recombinant viral structural and nonstructural proteins for investigation of virus life-cycle

·         Virus detection using antibodies- and/or DNA/RNA-based methods (LAMP, RT-LAMP, PCR, RT-PCR, qPCR)

Biotechnological utilization of plant viruses

·         Design of plant viral vectors based on several RNA and DNA plant viruses

·         Adaptation of plant viral vectors to GoldenBraid cloning system

·         Transient expression of heterologous proteins in plants using vectors derived from plant viruses

·         Peptide/epitope display on the surface of virus particles (electron microscopy)

Metabolic engineering of plant cells

·         Development of platform for expression and accumulation of authentic human glycoproteins such as antibodies in plants

·         Plant genome editing (Crispr, RNAi)

·         Protein expression using plant tissue cultures

Influence of abiotic stress on plant virus infection

·         Analysis of virus infection upon heat, drought and cold treatment of plants


Figure 1: Nicotiana benthamiana plant

with Potato virus X that systemically infects the plant

and expresses green fluorescent protein (GFP).

Figure 2: Nicotiana benthamiana plant

infected with modified geminiviral DNA vector

based on BeYDV that expresses coral red fluorescent protein (DsRed).



Our laboratory has fruitful international collaboration with teams from Danforth Plant Science Center and USDA, St. Louis, USA; Biopharming Research Unit, University of Cape Town, South Africa; VIB Ghent, Belgium and RWTH, Aachen, Germany.





Mgr. Tomáš Moravec Ph.D.

Tel: +420 225 106 822


Skills: immunochemical and electrophoretic methods, PCR techniques, molecular cloning, bioinformatics, electron microscopy



Assoc. Prof. Noemi Čeřovská, CSc.

Tel: +420 225 106 819


Skills: immunochemical, biochemical and molecular biological study of properties of plant viruses, biotechnological use of plant viruses



Mgr. Hana Hoffmeisterová, Ph.D.

Tel: +420 225 106 819


Skills: On maternity leave


Dr.rer.nat.Ing. Helena Plchová

Tel: +420 225 106 819


Skills: molecular biology, PCR techniques, molecular cloning, sequencing, yeast two-hybrid, bioinformatics, biotechnological use of plant viruses


RNDr. Naďa Wilhelmová, CSc.

Tel: +420 225 106 814


Skills: organelles isolation, photochemical activities, photochemical efficiency (PAM fluorimetry), ROS detection, oxidative damage in lipids, proteins, and nuclei (Comet assay), antioxidative defence enzymatic and non- enzymatic,  electrophoresis, zymograms



RNDr. Oldřich Navrátil, CSc.

Tel: +420 225 106 822


Skills: recombinant DNA techniques, potato resistence to pests, plant transgenosis


PharmDr. Zuzana Pobořilová, Ph.D.

Tel: +420 225 106 818


Skills: plant tissue culture techniques, protein expression, electrophoretic methods, fluorescent microscopy



Ing. Jakub Dušek

Tel: +420 225 106 822



Mgr. Kateřina Kratochvílová

Tel: +420 225 106 821




Ing. Jitka Svobodová

Tel: +420 225 106 820


Skills: methods of molecular biology, plant propagation


Lenka Kolčabová



Dagmar Cibochová

RNDr. Jitka Folwarczna, Ph.D.

Ing. Jan Fousek, Ph.D.

Renata Hadámková

Karolina Müllerová

RNDr. Petr Vaculík, Ph.D.


Current projects:

We are participating in 2 projects:


Centre for Experimental Plant Biology. Ministry of Education, Youth and Sports CZ.02.1.01/0.0/0.0/16_019/0000738; 2018-2023; principal investigator: R. Vaňková


Diamonds in the dust. Genetic basis of floral induction in the Chenopodium representatives with the contrasting photoperiodic response. Czech Science Foundation 19-01639S; 2019-2021; principal investigator: H. Štorchová


Past projects:

Impact of humanised glycosylation pathway on protein accumulation and traficking in plant seeds. Czech Science Foundation 501/15-10768S; 2015-2017; T. Moravec


Impact of different topology of heterologous antigenic determinants in PVX-based plant viral vector on their expression level and immunogenicity. Czech Science Foundation P501/12/1761; 2012-2014; N. Čeřovská


Biosafe plant virus expression system for transient production of human papillomavirus oncoproteins and their use for therapeutic vaccine development. Czech Science Foundation  521/09/1525; 2009-2011; N. Čeřovská


Variability of Potato leafroll luteovirus (PLRV), improved reliability of its detection and utilization of transgenic resistence. Ministry of Agriculture NAZV QH71123; 2007-2011; T. Moravec


Participation on Grant Ministry of Education, Youth and Sports 1M06030 „Genomics and proteomics in plant breeding“; 2006-2009; N. Čeřovská


Expression of  papillomavirus antigenic epitopes in plants using plant viral vector. Czech Science Foundation  521/06/0973; 2006-2008; N. Čeřovská



List of publications


Holá D., Benešová M., Fischer L., Haisel D., Hnilička F., Hniličková H., Jedelský P.L., Kočová M., Procházková D., Rothová O., Tůmová L., Wilhelmová N. The disadvantages of being a hybrid during drought: A combined analysis of plant morphology, physiology and leaf proteome in maize. PLoS ONE 12(4), e0176121 (2017)


Plchova H., Cerovska N., Vaculik P., Moravec T. Plant viruses as scaffolds for the presentation of vaccine epitopes. BIOLOGIA PLANTARUM 61: 1-12 (2017)



Vaculik P., Plchova H., Moravec T., Hoffmeisterova H., Cerovska N., Smahel M. Potato virus X displaying the E7 peptide derived from Human papillomavirus type 16: a novel position for epitope presentation. PLANT CELL, TISSUE AND ORGAN CULTURE 120: 671-680 (2015)


Plchova H., Vaculik P., Cerovska N., Moravec T., Dedic P. Molecular and biological analysis of Potato virus M (PVM) isolates from the Czech Republic. JOURNAL OF PHYTOPATHOLOGY 163: 1031 – 1035 (2015)


Vaculik P., Plchova H., Moravec T., Hoffmeisterova H., Cerovska N. New positions for peptide presentation in Potato virus X capsid protein. OPEN LIFE SCIENCES 10: 133-141 (2015)


Spoustová P., Hýsková V., Müller K., Schnablová R., Ryšlavá H., Čeřovská N., Malbeck J., Cvikrová M., Synková H. Tobacco susceptibility to Potato virus YNTN infection is affected by grafting and endogenous cytokinin content. PLANT SCIENCE 235: 25-36 (2015)


Janda M., Navrátil O., Haisel D., Jindřichová B., Fousek J., Burketová L., Čeřovská N., Moravec T. Growth and stress response in Arabidopsis thaliana, Nicotiana benthamiana, Glycine max, Solanum tuberosum and Brassica napus cultivated under polychromatic LEDs. PLANT METHODS 11:31 (2015)



Cerovska N., Plchova H., Vaculik P., Moravec T., Gichner T. Potato virus X induces DNA damage in leaf nuclei of the host tobacco plant Nicotiana tabacum L. cv. Xanthi. BIOLOGIA PLANTARUM 58: 783-787 (2014)


Moravec T., Cerovska N. The use of legume seed for expression and storage of high value proteins. CZECH JOURNAL OF GENETICS AND PLANT BREEDING 50: 69-76 (2014)



Cerovska N., Moravec T., Hoffmeisterova H., Plchova H., Synkova H., Polakova I., Duskova M., Smahel M. Expression of a recombinant Human papillomavirus 16 E6GT oncoprotein fused to N- and C-termini of Potato virus X coat protein in Nicotiana benthamiana. PLANT CELL, TISSUE AND ORGAN CULTURE 113: 81-90 (2013)  


Spoustová P., Synková H., Valcke R., Čeřovská N. Chlorophyll a fluorescence as a tool for a study of the Potato virus Y effects on photosynthesis of nontransgenic and transgenic Pssu-ipt tobacco. PHOTOSYNTHETICA 51: 191-201 (2013)   


Kodrík D., Kludkiewicz B., Navrátil O., Skoková Habuštová O., Horáčková V., Svobodová Z., Vinokurov K.S., Sehnal F. Protease inhibitor from insect silk - activities of derivatives expressed in vitro and in transgenic potato. APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY 171: 209-224 (2013).



Cerovska N., Hoffmeisterova H., Moravec T., Plchova H., Folwarczna J., Synkova H., Ryslava H., Ludvikova V., Smahel M. Transient expression of Human papillomavirus type 16 L2 epitope fused to N- and C-terminus of coat protein of Potato virus X in plants. JOURNAL OF BIOSCIENCES 37: 125-133 (2012)   


Hoffmeisterova H., Moravec T., Plchova H., Folwarczna J., Cerovska N. The influence of the N- and C- terminal modifications of Potato virus X coat protein on virus properties. BIOLOGIA PLANTARUM 56: 775-779 (2012) 


Cerovska N., Moravec T., Plchova H., Hoffmeisterova H., Dedic P. Production of polyclonal antibodies to the recombinant Potato virus M (PVM) nonstructural triple gene block protein 1 and coat protein. JOURNAL OF PHYTOPATHOLOGY 160: 251-254 (2012)  


Folwarczna J., Moravec T., Plchova H., Hoffmeisterova H., Cerovska N. Efficient expression of Human papillomavirus 16 E7 oncoprotein fused to C-terminus of Tobacco mosaic virus (TMV) coat protein using molecular chaperones in E. coli. PROTEIN EXPRESSION AND PURIFICATION 85: 152-157 (2012)   


Navrátil O., Kodrík D., Kludkiewicz B., Vinokurov K.S., Sehnal F., Horáčková V. Protease inhibitors as a possible new factor in agricultural plant protection against microbial and fungal attack. GMOs in INTEGRATED PLANT PRODUCTION IOBC/WPRS BULLETIN 73: 61-67 (2012) 


Navrátil O., Bucher P., Vacek J. Transgene coding of a key enzyme of the glycolytic pathway helps to decrease sugar content in potato tubers. CZECH JOURNAL OF GENETICS AND PLANT BREEDING 48: 42-45 (2012)  


Čeřovská N. Vakcíny pěstované v rostlinách - Budou se vakcíny pěstovat ve skleníku? VESMÍR 91: 594-596 (2012), in Czech   


Vacek J., Bucher P., Navrátil O. Effect of storage temperature and year on chips colour in transgenic lines of the potato variety Vladan. Conference Applied plant biotechnology (11.-13. September 2012 in Lednice) 9: 194-199 (2012)



Plchova H., Moravec T., Hoffmeisterova H., Folwarczna J., Cerovska N. Expression of Human papillomavirus 16 E7ggg oncoprotein on N- and C- terminus of Potato virus X coat protein in bacterial and plant cells. PROTEIN EXPRESSION AND PURIFICATION 77:  146-152 (2011) 


Plchova H., Moravec T., Dedic P., Cerovska N. Expression of Recombinant Potato leafroll virus Structural and Non-structural Proteins for Antibody Production. JOURNAL OF PHYTOPATHOLOGY 159: 130-132 (2011)



Cerovska N., Moravec T., Plchova H., Hoffmeisterova H., Folwarczna J., Dedic P. Production of polyclonal antibodies to Potato virus X (PVX) using recombinant coat protein. JOURNAL OF PHYTOPATHOLOGY 158: 66-68 (2010) 


Semenyuk E. G., Schmidt M. A., Beachy R. N., Moravec T., Woodford-Thomas T. Adaptation of an ecdysone-based genetic switch for transgene expression in soybean seeds. TRANSGENIC RESEARCH 19: 987–999 (2010)  



Plchova H., Cerovska N., Moravec T., Dedic P. Molecular analysis of Potato leafroll virus isolates from the Czech Republic. VIRUS GENES 39: 153-155 (2009)  


Müller K., Doubnerová V., Synková H., Čeřovská N., Ryšlavá H. Regulation of phosphoenolpyruvate carboxylase in PVYNTN-infected tobacco plants. BIOLOGICAL CHEMISTRY 390: 245-251 (2009)   


Doubnerová V., Muller K., Čeřovská N., Synková H., Spoustová P., Ryšlavá H.

Effect of Potato virus Y on the NADP-malic enzyme from Nicotiana tabacum L.: mRNA, expressed protein and aktivity. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES    10: 3583-3598 (2009)



Čeřovská N., Hoffmeisterová H., Pečenková T., Moravec T., Synková H., Plchová H., Velemínský J. Transient expression of HPV16 E7 peptide (aa 44-60) and HPV16 L2 peptide (aa 108-120) on chimeric potyvirus-like particles using Potato virus X-based vector. PROTEIN EXPRESSION AND PURIFICATION 58: 154-161 (2008) 


Čeřovská N., Hoffmeisterová H., Moravec T., Plchová H., Folwarczna J., Hadámková R. Optimum storage conditions for product of transiently expressed epitopes of Human papillomavirus using Potato virus X-based vector. BIOLOGIA PLANTARUM 52: 184-186  (2008)  


Hoffmeisterová H., Čeřovská N., Moravec T., Plchová H., Folwarczna J., Veleminsky J. Transient expression of fusion gene coding for the HPV-16 epitopes fused to the sequence of potyvirus coat protein using different means of inoculation of Nicotiana benthamiana and Brassica rapa, cv. Rapa plants. PLANT CELL, TISSUE AND ORGAN CULTURE 94: 261-267 (2008) 


Folwarczna J., Plchová H., Moravec T., Hoffmeisterová H., Dědič P., Čeřovská N. Production of polyclonal antibodies to a recombinant coat protein of Potato virus Y. FOLIA MICROBIOLOGICA 53: 438-442 (2008)

Doubnerová V., Janošková M., Synková H., Šubr Z., Čeřovská N., Ryšlavá H. Effect of Potato virus Y on activities of antioxidant and anaplerotic enzymes in Nicotiana tabacum l. transgenic plants with the gene for p3 protein. General and Applied Plant Physiology 33: 123-140 (2008)


Navratil O., Vacek J., Bucher P. Vliv exprese transgenu cukerného metabolizmu na obsah redukujících cukrů v hlízách. VĚDECKÉ PRÁCE - VÝZKUMNÝ ÚSTAV BRAMBORÁŘSKÝ HAVLÍČKůV BROD 16: 57-64 (2008), in Czech



Doubnerová V., Janoušková M., Synková H., Šubr Z., Čeřovská N., Ryšlavá H. Effect of Potato virus Y on activities of antioxidant and anaplerotic enzymes in Nicotina tabacum L. transgenic plants with the gene for p3 protein. GENERAL AND APPLIED PLANT PHYSIOLOGY 33: 123-140 (2007) 


Ryšlavá H., Doubnerová V., Müller K., Baťková P., Schnablová R., Liberda J., Synková H., Čeřovská N. The enzyme kinetics of the NADP-malic enzyme from tobacco leaves. COLLECTION OF CZECHOSLOVAK CHEMICAL COMMUNICATIONS 72: 1420-1434 (2007)


Čeřovská N., Pečenková T., Filigarová M., Dědič P. Sequence analysis of the Czech potato mop-top virus (PMTV) isolate Korneta-Nemilkov. FOLIA MICROBIOLOGICA 52: 61-64  (2007) 


Doubnerová V., Jirásková A., Janošková M., Müller K., Baťková P., Synková H., Čeřovská N., Ryšlavá H. The activity and isoforms of NADP-malic enzyme in Nicotiana benthamiana plants under biotic stress. GENERAL PHYSIOLOGY AND BIOPHYSICS 26: 281-289 (2007)  


Moravec T., Schmidt M.A., Herman E.M., Woodford-Thomas T. Production of Escherichia coli heat labile toxin (LT) B subunit in soybean seed and analysis of its immunogenicity as an oral vaccine. VACCINE 25: 1647-1657 (2007) 


Navrátil O., Fischer L., Čmejlová J., Linhart M., Vacek J. Decreased amount of reducing sugars in transgenic potato tubers and its influence on yield characteristics. BIOLOGIA PLANTARUM 51: 56-60 (2007)



Synková H., Semorádová H., Schnablová R., Muller K., Pospíšilová J., Ryšlavá H., Malbeck J., Čeřovská N. Effect of biotic stress caused by Potato virus Y on photosynthesis in ipt transgenic and control Nicotiana tabacum L. PLANT SCIENCE 171: 607-616 (2006)  


Čeřovská N., Filigarová M., Pečenková T. Production of polyclonal antiobies to a recombinant Potato mop-top virus non-structural Triple Gene Block Protein l. JOURNAL OF PHYTOPATHOLOGY 154: 422-427 (2006) 


Navrátil, O. Transgenóze rostlin. In Molekulární biologie a genetika XII. Praha: Ústav molekulární genetiky AV ČR 77-89 (2006), in Czech



Schnablová R., Synková H., Čeřovská N. The influence of Potato virus Y infection on the ultrastructure of Pssu-ipt transgenic tobacco. INTERNATIONAL JOURNAL OF PLANT SCIENCES 166: 713-721 (2005)   


Pokorná D., Čeřovská N., Šmahel M., Moravec T., Ludvíková V., Machková J., Synková H., Dušková M., Hozák P., Velemínský J. DNA vaccines based on chimeric potyvirus-like particles carrying HPV16 E7 peptide (aa 44-60). ONCOLOGY REPORTS 14: 1045-1053 (2005) 


Synková H., Schnablová R., Hušák M., Šiffel P., Bumba L., Hunalová I., Čeřovská N., Vácha F. Isolation and characterization of paracrystalline structures from transgenic Pssu-ipt tobacco. PHOTOSYNTHETICA 43: 509-517 (2005)   


Pečenková T., Filigarová M., Čeřovská N. Efficient bacterial expression of recombinant potato mop-top virus non-structural triple gene block protein 1 modified by progressive deletion of its N-terminus. PROTEIN EXPRESSION AND PURIFICATION 41: 128-135 (2005)


Nováková S., Mazurová L., Čeřovská N., Šubr Z.W.: Transgenic tobacco plants carrying the non-structural P3 gene of potato virus A. BIOLOGIA PLANTARUM 49: 593-598 (2005)



Čeřovská N., Pečenková T., Moravec T., Velemínský J. Transient expression of heterologous model gene in plants using Potato virusX-based vector. PLANT CELL, TISSUE AND ORGAN CULTURE 79: 147-152 (2004) 


Pečenková T., Moravec T., Filigarová M., Rosecká P., Čeřovská N. Extended Sequence Analysis of Three Danish Potato Mop-Top Virus (PMTV) Isolates. VIRUS GENES 29: 249-255 (2004) 



Čeřovská N., Moravec T., Rosecká P., Filigarová M., Pečenková T. Nucleotide sequences of coat protein coding regions of six potato mop-top virus isolates. ACTA VIROLOGICA 47: 37-40 (2003)


Čeřovská N., Moravec T., Rosecká P., Dědič P., Filigarová M. Production of Polyclonal Antibodies to a Recombinant Coat Protein of Potato mop-top virus. JOURNAL OF PHYTOPATHOLOGY 151: 195-200 (2003) 


Moravec T., Čeřovská N., Boonham N. The detection of recombinant, tuber necrosing isolates of Potato virus Y (PVYNTN) using a three-primer PCR based in the coat protein gene. JOURNAL OF VIROLOGICAL METHODS 109: 63-68 (2003) 


Ryšlavá H., Müller K., Semorádová Š., Synková H., Čeřovská N. Photosynthesis and activity of phosphoenolpyruvate carboxylase in Nicotiana tabacum L. leaves infected by Potato virus A and Potato virus Y. PHOTOSYNTHETICA 41: 357-363 (2003)


Plchova H., Hartung F., Puchta H. Biochemical characterization of an exonuclease from Arabidopsis thaliana reveals similarities to the DNA exonuclease of the human Werner syndrome protein. JOURNAL OF BIOLOGICAL CHEMISTRY 278: 44128-44138 (2003)



Čeřovská N., Filigarová M., Hadámková R. Optimum conditions for the storage of potato virus YNTN strain. BIOLOGIA PLANTARUM 45: 315-316 (2002)


Čeřovská N., Moravec T., Veleminský J. Polyclonal antibodies to a recombinant coat protein of Potato virus A. ACTA VIROLOGICA 46: 147-151 (2002)



Čeřovská N., Moravec T., Filigarová M., Petrzik K. Nucleotide sequences of 5´ terminal parts of coat protein genes of various isolates of NTN strain of Potato virus Y. ACTA VIROLOGICA 45: 55-59 (2001)


Čeřovská N., Moravec T., Veleminský J. Expression of Potato virus A (PVA) coat protein in Escherichia coli and possibility of its use as foreign antigen carrier. EUROPEAN JOURNAL OF BIOCHEMISTRY 268: 214 (2001)



Moravec T., Čerovská N. Rekombinantní protilátky scFv (single- chain variable fragments) - nový přístup k serologické detekci rostlinných pathogenů. BIOLOGICKÉ LISTY 65: 226-229 (2000), in Czech


Hartung F., Plchova H., Puchta H. Molecular characterisation of RecQ homologues in Arabidopsis thaliana. NUCLEIC ACIDS RESEARCH 28: 4275-4282 (2000)



Čeřovská N., Moravec T., Filigarová M., Ryšlavá H., Grosclaude J. Partial antigenic characterization of different potato virus Y-NTN isolates with monoclonal antibodies by means of competitive bindingb tests and immunoblot analysis. ACTA VIROLOGICA 43: 391-393 (1999)



Čeřovská N. Production of monoclonal antibodies to potato virus YNTN strain and their use in strain differentiation. PLANT PATHOLOGY 47: 505-509 (1998)


Čeřovská N., Filigarová M., Moravec T., Šubr Z. Mapping antigenic epitopes of potato virus A using monoclonal antibodies and overlapping synthetic peptides. CANADIAN JOURNAL OF PLANT PATHOLOGY 20: 221-226 (1998)


Čeřovská N., Petrzik K., Moravec T., Mráz I. Potato virus A detection by reverse transcription polymerase chain reaction. ACTA VIROLOGICA 42: 83-85 (1998)


Moravec T., Cerovska N., Pavlicek A. Electron microscopic observation of potato virus a using murine monoclonal antibodies. ACTA VIROLOGICA 42: 341-345 (1998)



Čeřovská N., Filigarová M., Šubr Z. Optimization of purification procedure for potato virus Y strain NN. ACTA VIROLOGICA 41: 47-49 (1997)



Čeřovská N., Filigarová M., Šubr Z. Partial antigenic characterization of potato virus S (Andean strain) by monoclonal antibodies. ACTA VIROLOGICA 40: 23-26 (1996)


Šubr Z., Moravec T., Čeřovská N. Monoclonal antibodies against potato virus A – Immnoblot analysis. ACTA VIROLOGICA 40: 289-292 (1996)


Moravec T., Čeřovská N. Effect of formaldehyde and glutaraldehyde fixation on the immunochemical reactivity of Potato virus A (PVA) with monoclonal antibodies. BIOLOGIA PLANTARUM 38:637-640 (1996)



Čeřovská N., Filigarová M. Optimum conditions for the storage of the potato virus S (PVS). ACTA VIROLOGICA 39: 51-52 (1995)


Čeřovská N., Filigarová M. Specific detection of the Andean strain of potato virus S by monoclonal antibodies. ANNALS OF APPLIED BIOLOGY 127: 87-93 (1995)


Gichner T., Stavreva D.A., Cerovska N., Wagner E.D., Plewa M.J. Metabolic-activation of m-phenylenediamine to products mutagenic in Salmonella-typhimurium by medium isolated from tobacco suspension cell-cultures. MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS 331: 127-132 (1995)