Research
About
Laboratory of Virology is focused on the study of
plant viruses and management of plant viral diseases, biotechnological utilization
of plant viruses, metabolic engineering of plant cells and very recently on
investigation of influence of abiotic stress on plant virus infection.
Our model
plant species are A. thaliana and N. benthamiana and the
leguminous crop plants soybean and pea (G. max and P.
sativum). We use not only whole plants but also tissue cultures.
For our
experiments we use several RNA and DNA plant viruses, namely Potato
virus X, Tobacco mosaic virus, Bean yellow dwarf virus and Cowpea
mosaic virus.
During the last three years, we have
introduced a novel modular cloning strategy based on the GoldenBraid system
that allows us to efficiently design genomic elements and complex plant
expression cassettes for metabolic engineering of plant cells.
Research areas of the laboratory:
Understanding and management of plant viral diseases
·
Study
of molecular properties of plant viruses by immunological and molecular
biological methods
·
Genome mapping of plant viruses, viral isolates and
phylogenetic analysis
·
Preparation of polyclonal antibodies against recombinant
viral structural and nonstructural proteins for investigation of virus
life-cycle
·
Virus detection using antibodies- and/or DNA/RNA-based
methods (LAMP, RT-LAMP, PCR, RT-PCR, qPCR)
Biotechnological utilization of plant viruses
·
Design of plant viral vectors based on several RNA and
DNA plant viruses
·
Adaptation of plant viral vectors to GoldenBraid cloning
system
·
Transient expression of heterologous proteins in plants
using vectors derived from plant viruses
·
Peptide/epitope display on the surface of virus particles
(electron microscopy)
Metabolic engineering of plant cells
·
Development of platform for expression and
accumulation of authentic human glycoproteins such as antibodies in plants
·
Plant
genome editing (Crispr, RNAi)
·
Protein
expression using plant tissue cultures
Influence of abiotic stress on plant virus infection
·
Analysis
of virus infection upon heat, drought and cold treatment of plants
 |
 |
Figure 1: Nicotiana benthamiana plant
with Potato virus X that systemically
infects the plant
and expresses green fluorescent protein (GFP). |
Figure 2: Nicotiana benthamiana plant
infected with modified geminiviral DNA vector
based on BeYDV that expresses coral red fluorescent protein (DsRed). |
Collaboration
Our laboratory has fruitful
international collaboration with teams from Danforth Plant Science Center and
USDA, St. Louis, USA; Biopharming Research Unit, University of Cape Town, South
Africa; VIB Ghent, Belgium and RWTH, Aachen, Germany.
People
HEAD
OF LABORATORY
Mgr. Tomáš Moravec Ph.D.
Tel: +420 225 106 822
E-Mail: moravec@ueb.cas.cz
Skills: immunochemical and electrophoretic methods, PCR
techniques, molecular cloning, bioinformatics, electron microscopy
SENIOR SCIENTISTS
Assoc. Prof. Noemi Čeřovská, CSc.
Tel:
+420 225 106 819
E-Mail: cerovska@ueb.cas.cz
Skills:
immunochemical, biochemical and molecular biological study of properties of
plant viruses, biotechnological use of plant viruses
SCIENTISTS
Mgr. Hana Hoffmeisterová, Ph.D.
Tel:
+420 225 106 819
E-Mail: cerovska@ueb.cas.cz
Skills: On maternity leave
Dr.rer.nat.Ing. Helena Plchová
Tel:
+420 225 106 819
E-Mail: plchova@ueb.cas.cz
Skills: molecular biology, PCR techniques, molecular
cloning, sequencing, yeast two-hybrid, bioinformatics, biotechnological
use of plant viruses
RNDr. Naďa Wilhelmová, CSc.
Tel:
+420 225 106 814
E-Mail: wilhelmova@ueb.cas.cz
Skills: organelles isolation, photochemical
activities, photochemical efficiency (PAM fluorimetry), ROS detection,
oxidative damage in lipids, proteins, and nuclei (Comet assay), antioxidative
defence enzymatic and non- enzymatic,
electrophoresis, zymograms
SCIENTIFIC ASSISTANT
RNDr. Oldřich Navrátil, CSc.
Tel:
+420 225 106 822
E-Mail: navratil@ueb.cas.cz
Skills: recombinant DNA techniques, potato resistence to
pests, plant transgenosis
POSTDOCTORAL
FELLOW
PharmDr. Zuzana Pobořilová, Ph.D.
Tel:
+420 225 106 818
E-Mail: poborilova@ueb.cas.cz
Skills: plant tissue culture
techniques, protein expression, electrophoretic methods, fluorescent microscopy
PhD
STUDENTS
Ing. Jakub Dušek
Tel:
+420 225 106 822
E-Mail: dusek@ueb.cas.cz
Mgr. Kateřina Kratochvílová
Tel:
+420 225 106 821
E-Mail:
kratochvilova@ueb.cas.cz
GRADUATED TECHNICAL ASSISTANT
Ing. Jitka Svobodová
Tel:
+420 225 106 820
E-Mail: svobodovaj@ueb.cas.cz
Skills: methods of molecular biology, plant propagation
TECHNICIANS
Lenka
Kolčabová
FORMER
MEMBERS OF LABORATORY
Dagmar Cibochová
RNDr. Jitka Folwarczna, Ph.D.
Ing. Jan Fousek, Ph.D.
Renata Hadámková
Karolina Müllerová
RNDr. Petr Vaculík, Ph.D.
Projects
Current projects:
We are participating in 2
projects:
Centre for Experimental Plant Biology. Ministry of Education, Youth and Sports CZ.02.1.01/0.0/0.0/16_019/0000738; 2018-2023; principal investigator: R. Vaňková
Diamonds
in the dust. Genetic basis of floral induction in the Chenopodium representatives
with the contrasting photoperiodic response. Czech Science Foundation 19-01639S;
2019-2021; principal investigator: H. Štorchová
Past projects:
Impact of humanised glycosylation pathway on protein accumulation and
traficking in plant seeds. Czech Science
Foundation 501/15-10768S; 2015-2017; T. Moravec
Impact of different topology of heterologous antigenic determinants in
PVX-based plant viral vector on their expression level and immunogenicity. Czech Science Foundation P501/12/1761; 2012-2014;
N. Čeřovská
Biosafe plant virus
expression system for transient production of human papillomavirus oncoproteins
and their use for therapeutic vaccine development. Czech Science Foundation 521/09/1525; 2009-2011; N. Čeřovská
Variability of
Potato leafroll luteovirus (PLRV), improved reliability of its detection and
utilization of transgenic resistence. Ministry of Agriculture NAZV QH71123;
2007-2011; T. Moravec
Participation
on Grant Ministry of Education, Youth and Sports 1M06030 „Genomics and proteomics
in plant breeding“; 2006-2009; N. Čeřovská
Expression
of papillomavirus antigenic epitopes in
plants using plant viral vector. Czech Science Foundation 521/06/0973; 2006-2008; N.
Čeřovská
List of
publications
2017
Holá D., Benešová
M., Fischer L., Haisel D., Hnilička F., Hniličková H., Jedelský P.L., Kočová
M., Procházková D., Rothová O., Tůmová L., Wilhelmová N. The disadvantages of
being a hybrid during drought: A combined analysis of plant morphology,
physiology and leaf proteome in maize. PLoS ONE 12(4), e0176121 (2017)
Plchova H., Cerovska
N., Vaculik P., Moravec T. Plant viruses as scaffolds for the presentation of
vaccine epitopes. BIOLOGIA PLANTARUM 61: 1-12 (2017)
2015
Vaculik P., Plchova H., Moravec T., Hoffmeisterova
H., Cerovska N., Smahel M. Potato virus X displaying the E7 peptide derived
from Human papillomavirus type 16: a novel position for epitope presentation.
PLANT CELL, TISSUE AND ORGAN CULTURE 120: 671-680 (2015)
Plchova H., Vaculik P., Cerovska N.,
Moravec T., Dedic P. Molecular and biological analysis of Potato virus M (PVM)
isolates from the Czech Republic. JOURNAL OF PHYTOPATHOLOGY 163: 1031 – 1035 (2015)
Vaculik P.,
Plchova H., Moravec T., Hoffmeisterova H., Cerovska N. New positions for peptide presentation in Potato virus
X capsid protein. OPEN LIFE SCIENCES 10: 133-141 (2015)
Spoustová P.,
Hýsková V., Müller K., Schnablová R., Ryšlavá H., Čeřovská N., Malbeck J.,
Cvikrová M., Synková H. Tobacco susceptibility to Potato virus YNTN infection
is affected by grafting and endogenous cytokinin content. PLANT SCIENCE 235: 25-36 (2015)
Janda M., Navrátil
O., Haisel D., Jindřichová B., Fousek J., Burketová L., Čeřovská N., Moravec T.
Growth and stress response in Arabidopsis thaliana, Nicotiana benthamiana,
Glycine max, Solanum tuberosum and Brassica napus cultivated under
polychromatic LEDs. PLANT METHODS 11:31 (2015)
2014
Cerovska N.,
Plchova H., Vaculik P., Moravec T., Gichner T. Potato virus X induces DNA
damage in leaf nuclei of the host tobacco plant Nicotiana tabacum L. cv.
Xanthi. BIOLOGIA PLANTARUM 58: 783-787 (2014)
Moravec T.,
Cerovska N. The use of legume seed for expression and storage of high value
proteins. CZECH JOURNAL OF GENETICS AND PLANT BREEDING 50: 69-76 (2014)
2013
Cerovska N.,
Moravec T., Hoffmeisterova H., Plchova H., Synkova H., Polakova I., Duskova M.,
Smahel M. Expression of a recombinant Human papillomavirus 16 E6GT oncoprotein
fused to N- and C-termini of Potato virus X coat protein in Nicotiana
benthamiana. PLANT CELL, TISSUE AND ORGAN CULTURE 113: 81-90 (2013)
Spoustová P.,
Synková H., Valcke R., Čeřovská N. Chlorophyll a fluorescence as a tool for a
study of the Potato virus Y effects on photosynthesis of nontransgenic and
transgenic Pssu-ipt tobacco. PHOTOSYNTHETICA 51: 191-201 (2013)
Kodrík D.,
Kludkiewicz B., Navrátil O., Skoková Habuštová O., Horáčková V., Svobodová Z.,
Vinokurov K.S., Sehnal F. Protease inhibitor from insect silk - activities of
derivatives expressed in vitro and in transgenic potato. APPLIED BIOCHEMISTRY
AND BIOTECHNOLOGY 171: 209-224 (2013).
2012
Cerovska N.,
Hoffmeisterova H., Moravec T., Plchova H., Folwarczna J., Synkova H., Ryslava
H., Ludvikova V., Smahel M. Transient expression of Human papillomavirus type
16 L2 epitope fused to N- and C-terminus of coat protein of Potato virus X in
plants. JOURNAL OF BIOSCIENCES 37: 125-133 (2012)
Hoffmeisterova
H., Moravec T., Plchova H., Folwarczna J., Cerovska N. The influence of the N-
and C- terminal modifications of Potato virus X coat protein on virus
properties. BIOLOGIA PLANTARUM 56: 775-779 (2012)
Cerovska N.,
Moravec T., Plchova H., Hoffmeisterova H., Dedic P. Production of polyclonal
antibodies to the recombinant Potato virus M (PVM) nonstructural triple gene
block protein 1 and coat protein. JOURNAL OF PHYTOPATHOLOGY 160: 251-254
(2012)
Folwarczna J.,
Moravec T., Plchova H., Hoffmeisterova H., Cerovska N. Efficient expression of
Human papillomavirus 16 E7 oncoprotein fused to C-terminus of Tobacco mosaic
virus (TMV) coat protein using molecular chaperones in E. coli. PROTEIN
EXPRESSION AND PURIFICATION 85: 152-157 (2012)
Navrátil O.,
Kodrík D., Kludkiewicz B., Vinokurov K.S., Sehnal F., Horáčková V. Protease
inhibitors as a possible new factor in agricultural plant protection against
microbial and fungal attack. GMOs in INTEGRATED PLANT PRODUCTION IOBC/WPRS
BULLETIN 73: 61-67 (2012)
Navrátil O.,
Bucher P., Vacek J. Transgene coding of a key enzyme of the glycolytic pathway
helps to decrease sugar content in potato tubers. CZECH JOURNAL OF GENETICS AND
PLANT BREEDING 48: 42-45 (2012)
Čeřovská N.
Vakcíny pěstované v rostlinách - Budou se vakcíny pěstovat ve skleníku? VESMÍR
91: 594-596 (2012), in Czech
Vacek J.,
Bucher P., Navrátil O. Effect of storage temperature and year on chips colour
in transgenic lines of the potato variety Vladan. Conference Applied plant
biotechnology (11.-13. September
2011
Plchova H.,
Moravec T., Hoffmeisterova H., Folwarczna J., Cerovska N. Expression of Human
papillomavirus 16 E7ggg oncoprotein on N- and C- terminus of Potato virus X
coat protein in bacterial and plant cells. PROTEIN EXPRESSION AND PURIFICATION
77: 146-152 (2011)
Plchova H.,
Moravec T., Dedic P., Cerovska N. Expression of Recombinant Potato leafroll
virus Structural and Non-structural Proteins for Antibody Production. JOURNAL
OF PHYTOPATHOLOGY 159: 130-132 (2011)
2010
Cerovska N.,
Moravec T., Plchova H., Hoffmeisterova H., Folwarczna J., Dedic P. Production
of polyclonal antibodies to Potato virus X (PVX) using recombinant coat
protein. JOURNAL OF PHYTOPATHOLOGY 158: 66-68 (2010)
Semenyuk E. G.,
Schmidt M. A., Beachy R. N., Moravec T., Woodford-Thomas T. Adaptation of an
ecdysone-based genetic switch for transgene expression in soybean seeds.
TRANSGENIC RESEARCH 19: 987–999 (2010)
2009
Plchova H.,
Cerovska N., Moravec T., Dedic P. Molecular analysis of Potato leafroll virus
isolates from the Czech Republic. VIRUS GENES 39: 153-155 (2009)
Müller K.,
Doubnerová V., Synková H., Čeřovská N., Ryšlavá H. Regulation of
phosphoenolpyruvate carboxylase in PVYNTN-infected tobacco plants. BIOLOGICAL
CHEMISTRY 390: 245-251 (2009)
Doubnerová V.,
Muller K., Čeřovská N., Synková H., Spoustová P., Ryšlavá H.
Effect of
Potato virus Y on the NADP-malic enzyme from Nicotiana tabacum L.: mRNA,
expressed protein and aktivity. INTERNATIONAL JOURNAL OF MOLECULAR
SCIENCES 10: 3583-3598 (2009)
2008
Čeřovská N.,
Hoffmeisterová H., Pečenková T., Moravec T., Synková H., Plchová H., Velemínský
J. Transient expression of HPV16 E7 peptide (aa 44-60) and HPV16 L2 peptide (aa
108-120) on chimeric potyvirus-like particles using Potato virus X-based
vector. PROTEIN EXPRESSION AND PURIFICATION 58: 154-161 (2008)
Čeřovská N.,
Hoffmeisterová H., Moravec T., Plchová H., Folwarczna J., Hadámková R. Optimum
storage conditions for product of transiently expressed epitopes of Human
papillomavirus using Potato virus X-based vector. BIOLOGIA PLANTARUM 52:
184-186 (2008)
Hoffmeisterová
H., Čeřovská N., Moravec T., Plchová H., Folwarczna J., Veleminsky J. Transient
expression of fusion gene coding for the HPV-16 epitopes fused to the sequence
of potyvirus coat protein using different means of inoculation of Nicotiana
benthamiana and Brassica rapa, cv. Rapa plants. PLANT CELL, TISSUE AND ORGAN
CULTURE 94: 261-267 (2008)
Folwarczna J.,
Plchová H., Moravec T., Hoffmeisterová H., Dědič P., Čeřovská N. Production of
polyclonal antibodies to a recombinant coat protein of Potato virus Y. FOLIA
MICROBIOLOGICA 53: 438-442 (2008)
Doubnerová V., Janošková M., Synková H., Šubr Z., Čeřovská N., Ryšlavá H. Effect of Potato virus Y on activities of antioxidant and anaplerotic enzymes
in Nicotiana tabacum l. transgenic
plants with the gene for p3 protein. General and Applied Plant Physiology 33: 123-140 (2008)
Navratil O.,
Vacek J., Bucher P. Vliv exprese transgenu cukerného metabolizmu na obsah
redukujících cukrů v hlízách. VĚDECKÉ PRÁCE - VÝZKUMNÝ ÚSTAV BRAMBORÁŘSKÝ
HAVLÍČKůV BROD 16: 57-64 (2008), in Czech
2007
Doubnerová V.,
Janoušková M., Synková H., Šubr Z., Čeřovská N., Ryšlavá H. Effect of Potato
virus Y on activities of antioxidant and anaplerotic enzymes in Nicotina
tabacum L. transgenic plants with the gene for p3 protein. GENERAL AND APPLIED
PLANT PHYSIOLOGY 33: 123-140 (2007)
Ryšlavá H.,
Doubnerová V., Müller K., Baťková P., Schnablová R., Liberda J., Synková H.,
Čeřovská N. The enzyme kinetics of the NADP-malic enzyme from tobacco leaves.
COLLECTION OF CZECHOSLOVAK CHEMICAL COMMUNICATIONS 72: 1420-1434 (2007)
Čeřovská N.,
Pečenková T., Filigarová M., Dědič P. Sequence analysis of the Czech potato
mop-top virus (PMTV) isolate Korneta-Nemilkov. FOLIA MICROBIOLOGICA 52:
61-64 (2007)
Doubnerová V.,
Jirásková A., Janošková M., Müller K., Baťková P., Synková H., Čeřovská N.,
Ryšlavá H. The activity and isoforms of NADP-malic enzyme in Nicotiana
benthamiana plants under biotic stress. GENERAL PHYSIOLOGY AND BIOPHYSICS 26:
281-289 (2007)
Moravec T.,
Schmidt M.A., Herman E.M., Woodford-Thomas T. Production of Escherichia coli
heat labile toxin (LT) B subunit in soybean seed and analysis of its
immunogenicity as an oral vaccine. VACCINE 25: 1647-1657 (2007)
Navrátil O.,
Fischer L., Čmejlová J., Linhart M., Vacek J. Decreased amount of reducing
sugars in transgenic potato tubers and its influence on yield characteristics.
BIOLOGIA PLANTARUM 51: 56-60 (2007)
2006
Synková H.,
Semorádová H., Schnablová R., Muller K., Pospíšilová J., Ryšlavá H., Malbeck
J., Čeřovská N. Effect of biotic stress caused by Potato virus Y on
photosynthesis in ipt transgenic and control Nicotiana tabacum L. PLANT SCIENCE
171: 607-616 (2006)
Čeřovská N.,
Filigarová M., Pečenková T. Production of polyclonal antiobies to a recombinant
Potato mop-top virus non-structural Triple Gene Block Protein l. JOURNAL OF
PHYTOPATHOLOGY 154: 422-427 (2006)
Navrátil, O.
Transgenóze rostlin. In Molekulární biologie a genetika XII. Praha: Ústav
molekulární genetiky AV ČR 77-89 (2006), in Czech
2005
Schnablová R.,
Synková H., Čeřovská N. The influence of Potato virus Y infection on the
ultrastructure of Pssu-ipt transgenic tobacco. INTERNATIONAL JOURNAL OF PLANT
SCIENCES 166: 713-721 (2005)
Pokorná D.,
Čeřovská N., Šmahel M., Moravec T., Ludvíková V., Machková J., Synková H.,
Dušková M., Hozák P., Velemínský J. DNA vaccines based on chimeric
potyvirus-like particles carrying HPV16 E7 peptide (aa 44-60). ONCOLOGY REPORTS
14: 1045-1053 (2005)
Synková H.,
Schnablová R., Hušák M., Šiffel P., Bumba L., Hunalová I., Čeřovská N., Vácha
F. Isolation and characterization of paracrystalline structures from transgenic
Pssu-ipt tobacco. PHOTOSYNTHETICA 43: 509-517 (2005)
Pečenková T.,
Filigarová M., Čeřovská N. Efficient bacterial expression of recombinant potato
mop-top virus non-structural triple gene block protein 1 modified by
progressive deletion of its N-terminus. PROTEIN EXPRESSION AND PURIFICATION 41:
128-135 (2005)
Nováková S., Mazurová L.,
Čeřovská N., Šubr Z.W.: Transgenic tobacco plants carrying the non-structural
P3 gene of potato virus A. BIOLOGIA PLANTARUM 49: 593-598 (2005)
2004
Čeřovská N.,
Pečenková T., Moravec T., Velemínský J. Transient expression of heterologous
model gene in plants using Potato virusX-based vector. PLANT CELL, TISSUE AND
ORGAN CULTURE 79: 147-152 (2004)
Pečenková T.,
Moravec T., Filigarová M., Rosecká P., Čeřovská N. Extended Sequence Analysis
of Three Danish Potato Mop-Top Virus (PMTV) Isolates. VIRUS GENES 29: 249-255
(2004)
2003
Čeřovská N.,
Moravec T., Rosecká P., Filigarová M., Pečenková T. Nucleotide sequences of
coat protein coding regions of six potato mop-top virus isolates. ACTA
VIROLOGICA 47: 37-40 (2003)
Čeřovská N.,
Moravec T., Rosecká P., Dědič P., Filigarová M. Production of Polyclonal
Antibodies to a Recombinant Coat Protein of Potato mop-top virus. JOURNAL OF
PHYTOPATHOLOGY 151: 195-200 (2003)
Moravec T.,
Čeřovská N., Boonham N. The detection of recombinant, tuber necrosing isolates
of Potato virus Y (PVYNTN) using a three-primer PCR based in the coat protein
gene. JOURNAL OF VIROLOGICAL METHODS 109: 63-68 (2003)
Ryšlavá H.,
Müller K., Semorádová Š., Synková H., Čeřovská N. Photosynthesis and activity
of phosphoenolpyruvate carboxylase in Nicotiana tabacum L. leaves infected by
Potato virus A and Potato virus Y. PHOTOSYNTHETICA 41: 357-363 (2003)
Plchova H.,
Hartung F., Puchta H. Biochemical characterization of an exonuclease from
Arabidopsis thaliana reveals similarities to the DNA exonuclease of the human
Werner syndrome protein. JOURNAL OF BIOLOGICAL CHEMISTRY 278: 44128-44138
(2003)
2002
Čeřovská N.,
Filigarová M., Hadámková R. Optimum conditions for the storage of potato virus
YNTN strain. BIOLOGIA PLANTARUM 45: 315-316 (2002)
Čeřovská N.,
Moravec T., Veleminský J. Polyclonal antibodies to a recombinant coat protein
of Potato virus A. ACTA VIROLOGICA 46: 147-151 (2002)
2001
Čeřovská N., Moravec T., Filigarová M., Petrzik K. Nucleotide sequences of 5´ terminal parts of coat
protein genes of various isolates of NTN strain of Potato virus Y. ACTA
VIROLOGICA 45: 55-59 (2001)
Čeřovská N., Moravec T., Veleminský J. Expression of Potato virus A (PVA) coat protein in Escherichia coli
and possibility of its use as foreign antigen carrier. EUROPEAN JOURNAL OF BIOCHEMISTRY 268: 214 (2001)
2000
Moravec T.,
Čerovská N. Rekombinantní protilátky scFv (single- chain variable fragments) -
nový přístup k serologické detekci rostlinných pathogenů. BIOLOGICKÉ LISTY 65:
226-229 (2000), in Czech
Hartung F.,
Plchova H., Puchta H. Molecular characterisation of RecQ homologues in
Arabidopsis thaliana. NUCLEIC ACIDS RESEARCH 28: 4275-4282 (2000)
1999
Čeřovská N., Moravec T., Filigarová M., Ryšlavá H., Grosclaude J. Partial antigenic characterization of different
potato virus Y-NTN isolates with monoclonal antibodies by means of competitive
bindingb tests and immunoblot analysis. ACTA VIROLOGICA 43: 391-393 (1999)
1998
Čeřovská N. Production of
monoclonal antibodies to potato virus YNTN strain and their use in
strain differentiation. PLANT PATHOLOGY 47: 505-509 (1998)
Čeřovská N., Filigarová M., Moravec T., Šubr Z. Mapping antigenic epitopes of potato virus A using
monoclonal antibodies and overlapping synthetic peptides. CANADIAN JOURNAL OF
PLANT PATHOLOGY 20: 221-226
(1998)
Čeřovská N., Petrzik K., Moravec T., Mráz I. Potato virus A detection by reverse transcription
polymerase chain reaction. ACTA VIROLOGICA 42: 83-85 (1998)
Moravec T.,
Cerovska N., Pavlicek A. Electron microscopic observation of potato virus a
using murine monoclonal antibodies. ACTA VIROLOGICA 42: 341-345 (1998)
1997
Čeřovská N., Filigarová M., Šubr Z. Optimization of purification procedure for potato virus Y strain NN. ACTA
VIROLOGICA 41: 47-49 (1997)
1996
Čeřovská N., Filigarová M., Šubr Z. Partial antigenic characterization of potato virus S (Andean strain) by
monoclonal antibodies. ACTA VIROLOGICA 40: 23-26 (1996)
Šubr Z., Moravec T., Čeřovská N. Monoclonal antibodies against potato virus A – Immnoblot analysis. ACTA
VIROLOGICA 40: 289-292 (1996)
Moravec T., Čeřovská N. Effect of
formaldehyde and glutaraldehyde fixation on the immunochemical reactivity of
Potato virus A (PVA) with monoclonal antibodies. BIOLOGIA PLANTARUM 38:637-640
(1996)
1995
Čeřovská N., Filigarová M. Optimum conditions for the storage of the potato virus S (PVS). ACTA
VIROLOGICA 39: 51-52 (1995)
Čeřovská N., Filigarová M. Specific detection of the Andean strain of potato virus S by monoclonal
antibodies. ANNALS OF APPLIED BIOLOGY 127: 87-93 (1995)
Gichner T.,
Stavreva D.A., Cerovska N., Wagner E.D., Plewa M.J. Metabolic-activation of
m-phenylenediamine to products mutagenic in Salmonella-typhimurium by medium
isolated from tobacco suspension cell-cultures. MUTATION RESEARCH-FUNDAMENTAL
AND MOLECULAR MECHANISMS OF MUTAGENESIS 331: 127-132 (1995)